Positive IHC detected in: human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in: hTERT-RPE1 cells, MDCK cells
Recommended dilution: WB : 1:300-1:800 IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB IHC : 1:20-1:200 IF : 1:20-1:200 Product Information
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Immunogen Information
Full name: intraflagellar transport 20 homolog (Chlamydomonas)
Calculated molecular weight: 15 kDa
Observed molecular weight: 15-18 kDa
Gene symbol IFT20
Synonyms hIFT20, IFT20 Background Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium. IFT particles are multi-subunit complexes that are made up of complex A and complex B. IFT20 is a component of IFT complex B and involved in ciliary process assembly. It is associated with the Golgi complex and plays a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium.
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