Positive IHC detected in: human pancreas tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in: PC-3 cells
Recommended dilution: WB : 1:300-1:1000 IHC : 1:50-1:500 IF : 1:20-1:200 Product Information
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Immunogen Information
Full name: protein phosphatase 1, regulatory (inhibitor) subunit 15A
Calculated molecular weight: 73 kDa
Observed molecular weight: 100 kDa
Gene symbol PPP1R15A
Synonyms GADD34, growth arrest and DNA damage-inducible protein 34, PPP1R15A Background GADD34, also named PPP1R15A, belongs to the PPP1R15 family. GADD34 can be triggered as a direct target of activating transcription factor4 (ATF4) under ER stress, it plays a pivotal role in the recovery of cells from shut-down of translation induced by ER stress. It recruits the serine/threonine-protein phosphatase (PP1) to dephosphorylate the translation initiation factor eIF2alpha, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. GADD34 down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 via PP1. It may also promote apoptosis by inducing TP53 phosphorylation on ‘Ser-15’. Starvation-induced expression of GADD34 reduced mTOR activity and induced autophagy in wild-type mice, but not in GADD34 KO mice. Molecular weight of GADD34 is 100 kDa confirmed in GADD34 KO mice, and Proteintech’s GADD34 antibody 10449-1-AP primarily recongize the 100 kDa band.
杭州浩克生物
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