Cited Applications: FC, IF, IHC, WB Species Specificity: human
Cited Species: bovine, human, rabbit
Positive WB detected in: HL-60 cells
Positive IHC detected in: human tonsillitis tissue, human small intestine tissue, human gliomas tissue, human hysteromyoma tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in: human tonsillitis tissue, human liver cancer tissue
Recommended dilution: WB : 1:500-1:1000 IHC : 1:300-1:1200 IF : 1:50-1:500 Product Information
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Immunogen Information
Full name: CD34 molecule
Calculated molecular weight: 41 kDa
Observed molecular weight: 105-120 kDa
Gene symbol CD34
Synonyms CD34, CD34 molecule Background CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. This antibody 14486-1-AP is raised against 33-286 amino acids of full protein.
杭州浩克生物
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