Positive IHC detected in: human breast cancer tissue, human liver cancer tissue, mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in: HeLa cells, HepG2 cells
Positive FC detected in: HepG2 cells, HeLa cells, A549 cells
Recommended dilution: WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB IHC : 1:50-1:500 IF : 1:50-1:500 Product Information
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Immunogen Information
Full name: heat shock 70kDa protein 1A
Calculated molecular weight: 70 kDa
Observed molecular weight: 66-70 kDa
Gene symbol HSPA1A
Synonyms Heat shock 70 kDa protein 1/2, heat shock 70kDa protein 1A, HSP70, HSP70 1, HSP70 1/HSP70 2, HSP70 1A, HSP70.1/HSP70.2, HSP70I, HSP72, HSPA1, HSPA1A, HSPA1B Background HSPA1A , collectively known as HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. HSP70 is a useful marker for exosome. This antibody well recognized the endogenous HSP70 protein in multiple cell lines. (21373891)
杭州浩克生物
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