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GRP75兔多克隆抗体

GRP75兔多克隆抗体

一抗 抗体 试剂
  • 价格￥1200
  • 规格50ul
  • 货号HK210165S
  • 单位支
  • 品牌HaoKebio
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产品参数
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HaoKebioHK210165SGRP75兔多克隆抗体50ul支1200

Applications

Tested Applications:
FC, IF, IHC, IP, WB,ELISA

Cited Applications:
ICC/IF, IF, WB
Species Specificity:
human, mouse, rat

Cited Species:
human, mouse

Positive WB detected in:
HT-29 cells, A549 cells, rat testis tissue, mouse testis tissue, HeLa cells, Jurkat cells, SW480 cells

Positive IP detected in:
mouse brain tissue, NIH/3T3 cells

Positive IHC detected in:
human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF detected in:
HepG2 cells

Positive FC detected in:
HepG2 cells

Recommended dilution:
WB : 1:2000-1:10000
IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
IHC : 1:1000-1:4000
IF : 1:10-1:100
Product Information

Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
Immunogen Information

Full name:
heat shock 70kDa protein 9 (mortalin)

Calculated molecular weight:
74 kDa

Observed molecular weight:
74-75 kDa

Gene symbol
HSPA9

Synonyms
CSA, GRP 75, GRP75, Heat shock 70 kDa protein 9, HSPA9, HSPA9B, Mortalin, MOT, mot 2, MOT2, MTHSP75, PBP74, Peptide binding protein 74
Background
GRP75 (also known as mortalin, HSPA9 or mt-Hsp70) is a constitutively expressed member of the HSPA (HSP70) family of heat-shock proteins. It is located in the mitochondrial matrix and anti-GRP75 is commonly used as the marker for mitochondria. It has been reported that GRP75 is enriched in cancer cells and contributes to carcinogenesis. In addition, decreased expression level of GRP75 has been found in neurodegenerative disorders like Parkinson’s disease. This antibody recognized the endogenous GRP75 protein in NIH/3T3 cells. (PMID: 21640711, 22920904)

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