Tested Applications: FC, IF, IHC, IP, WB,ELISA Cited Applications: IF, IHC, IP, WB Species Specificity: human
Cited Species: human, monkey
Positive WB detected in: Jurkat cells
Positive IP detected in: Jurkat cells
Positive IHC detected in: human hepatocirrhosis tissue, human tonsillitis tissue, human liver cancer tissue, human gliomas tissue, human renal cell carcinoma tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in: human tonsillitis tissue
Positive FC detected in: HUVEC cells
Recommended dilution: WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB IHC : 1:800-1:4000 IF : 1:50-1:500 Product Information
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Immunogen Information
Full name: platelet/endothelial cell adhesion molecule
Calculated molecular weight: 83 kDa
Observed molecular weight: 130 kDa
Gene symbol PECAM1
Synonyms CD31, EndoCAM, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker Background Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).
杭州浩克生物
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