Positive IHC detected in: mouse kidney tissue, human breast cancer tissue, human pancreas cancer tissue, mouse heart tissue, rat brain tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in: HepG2 cells
Positive FC detected in: HeLa cells
Recommended dilution: WB : 1:1000-1:6000 IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB IHC : 1:50-1:500 IF : 1:50-1:500 Product Information
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Immunogen Information
Full name: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Calculated molecular weight: 78 kDa
Observed molecular weight: 70-78 kDa
Gene symbol HSPA5
Synonyms BIP, GRP 78, GRP78, HSP A5, HSPA5, MIF2 Background GRP78 (HSPA5), also referred to as ‘immunoglobulin heavy chain-binding protein’ (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth. 11587-1-AP antibody recognizes one or two bands with MW 70-78 kDa for the signal peptide of GRP78 protein can be finally cleaved.
杭州浩克生物
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