Positive IHC detected in: human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution: WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB IHC : 1:200-1:800 Product Information
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Immunogen Information
Full name: KIAA0831
Calculated molecular weight: 55 kDa
Observed molecular weight: 55 and 65 kDa
Gene symbol KIAA0831
Synonyms ATG14, Atg14L, Barkor, KIAA0831 Background ATG14 plays a critical role in autophagy which is required for both basal and inducible autophagy. It has been reported that the loss of ATG14 completely blocks autophagic fusion with lysosomes. ATG14 is associated with the location of PI3-kinase complex PI3KC3-C1 and the formation of autophagosome. Besides, BECN2 can form the BECN2:ATG14 heterodimer with ATG14. 28021-1-AP antibody recognizes the 55 kDa protein and the phosphorylated 65 kDa protein in SDS-PAGE. (PMID: 28218432, 24597603, 30894050)
杭州浩克生物
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