Positive WB detected in: human bladder tissue, UV treated HEK-293
Positive IP detected in: HEK-293 cells
Positive IHC detected in: human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution: WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB IHC : 1:20-1:200 Product Information
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Immunogen Information
Full name: ATG12 autophagy related 12 homolog (S. cerevisiae)
Calculated molecular weight: 16 kDa
Observed molecular weight: 48-50 kDa
Gene symbol ATG12
Synonyms APG12, APG12 like, APG12L, ATG12, Autophagy related protein 12, FBR93, HAPG12, Ubiquitin like protein ATG12 Background ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.
杭州浩克生物
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