Positive IHC detected in: human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in: HepG2 cells
Recommended dilution: WB : 1:500-1:1000 IHC : 1:50-1:500 IF : 1:10-1:100 Product Information
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Immunogen Information
Full name: autophagy/beclin-1 regulator 1
Calculated molecular weight: 1208 aa, 133 kDa
Observed molecular weight: 130-135 kDa
Gene symbol AMBRA1
Synonyms AMBRA1, autophagy/beclin 1 regulator 1, KIAA1736, WDR94 Background AMBRA1 (Activating molecule in BECN1-regulated autophagy protein 1), also known as WDR94 or KIAA1736, is a 1,298 amino acid protein that contains three WD repeats. AMBRA1 regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. This antibody works well in WB, IHC and IF application.
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