Positive WB detected in: HT-29 cells, rat brain tissue, mouse brain tissue, K-562 cells, HepG2 cells, HeLa cells, HT-29 cells
Positive IP detected in: mouse brain tissue
Positive IHC detected in: human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in: HepG2 cells
Positive FC detected in: K-562 cells
Recommended dilution: WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB IHC : 1:50-1:500 IF : 1:50-1:500 Product Information
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Immunogen Information
Synonyms ADP ribosylation factor 4, ARF2, ARF4 Background ARF4, also named as ARF2, belongs to the small GTPase superfamily and Arf family. ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. Their function are not fully described. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.
杭州浩克生物
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