Cited Applications: IF, IHC, IP, WB Species Specificity: human
Cited Species: human, mouse, Trypanosoma brucei
Positive WB detected in: human blood tissue, A549 cells, SKOV-3 cells, HeLa cells, MCF-7 cells, HepG2 cells, A2780 cells, SW 1990 cells, human plasma tissue
Positive IP detected in: HepG2 cells, human plasma tissue
Positive IHC detected in: human kidney tissue, human spleen tissue, human liver tissue, human pancreas tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in: HepG2 cells
Positive FC detected in: HepG2 cells
Recommended dilution: WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB IHC : 1:20-1:200 IF : 1:10-1:100 Product Information
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Immunogen Information
Full name: apolipoprotein L, 1
Calculated molecular weight: 44 kDa
Observed molecular weight: 39 kDa
Gene symbol APOL1
Synonyms APO L, APOL, APOL I, APOL1, Apolipoprotein L, Apolipoprotein L I, apolipoprotein L, 1, Apolipoprotein L1 Background Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue. This antibody recognizes the endogenous APOL1 of 39-45 kDa in blood lysate.
杭州浩克生物
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