Positive IHC detected in: human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in: A549 cells
Recommended dilution: WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB IHC : 1:50-1:500 IF : 1:20-1:200 Product Information
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Immunogen Information
Full name: apoptotic peptidase activating factor 1
Calculated molecular weight: 1248 aa, 142 kDa
Observed molecular weight: 130-142 kDa
Gene symbol APAF1
Synonyms APAF 1, APAF1, CED4, KIAA0413 Background APAF1, also named as Apoptotic protease-activating factor 1 or KIAA0413, is a 1248 amino acid protein, which contains one CARD domain, contains one NB-ARC domain and Contains fifteen WD repeats. APAF1 localizes in the cytoplasm and is widely expressed in many tissues. Highest levels of expression is in adult spleen, peripheral blood leukocytes, in fetal brain, kidney and lung. Oligomeric APAF1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.
杭州浩克生物
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