Cited Applications: IF, IHC, WB Species Specificity: human, mouse, rat
Cited Species: human, mouse, rat, sheep
Positive WB detected in: HeLa cells, Jurkat cells, A549 cells, rat kidney tissue, NIH/3T3 cells
Positive IP detected in: HeLa cells
Positive IHC detected in: human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in: HepG2 cells
Recommended dilution: WB : 1:1000-1:8000 IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB IHC : 1:100-1:400 IF : 1:10-1:100 Product Information
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Immunogen Information
Full name: apoptosis-inducing factor, mitochondrion-associated, 1
Calculated molecular weight: 609 aa, 66 kDa
Observed molecular weight: 67 kDa, 57 kDa
Gene symbol AIFM1
Synonyms AIF, AIFM1, PDCD8 Background Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).
杭州浩克生物
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