Cited Applications: CoIP, IF, IHC, IP, WB Species Specificity: human, mouse, rat
Cited Species: human, rat
Positive WB detected in: MCF-7 cells, PC-3 cells, A375 cells, HepG2 cells, HeLa cells, RAW 264.7 cells, mouse spleen tissue, DU 145 cells, A549 cells, HL-60 cells
Positive IP detected in: MCF-7 cells
Positive IHC detected in: human breast cancer tissue, mouse brain tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in: MCF-7 cells
Recommended dilution: WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB IHC : 1:200-1:800 IF : 1:20-1:200 Product Information
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Immunogen Information
Full name: metadherin
Calculated molecular weight: 582 aa, 64 kDa
Observed molecular weight: 65-70 kDa
Gene symbol MTDH
Synonyms 3D3, 3D3/LYRIC, AEG 1, AEG1, LYRIC, LYRIC/3D3, metadherin, Metastasis adhesion protein, MTDH, Protein LYRIC Background MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression. This antibody got two bands about 70 kDa and 80 kDa in western blotting, possibly due to posttranslational modification. This antibody is specific to AEG-1/MTDH.
杭州浩克生物
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