Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue usingHK210002 (smooth muscle actin antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescent analysis of (-20℃ Ethanol) fixed C2C12 cell using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide usingHK210002 (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Immunohistochemical analysis of paraffin-embedded human colon tissue slide using HK210002 (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Immunohistochemical analysis of paraffin-embedded human heart tissue slide using HK210002 (ACTA2/smooth muscle actin antibody at dilution of 1:5000 (under 10x lens). Various lysates were subjected to SDS PAGE followed by western blot withHK210002(smooth muscle actin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. IP Result of anti-ACTA2/smooth muscle actin (IP:14395-1-AP, 3ug; Detection:HK210002 1:1000) with mouse liver tissue lysate 6000ug. 1X10^6 C2C12 cell were stained with 0.2ug ACTA2/smooth muscle actin antibody (HK210002 , red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.
Species Specificity: human, mouse, rat
Cited Species: human, monkey, mouse, pig, rabbit, rat
Positive IHC detected in: human liver cancer tissue, human heart tissue, stromal tumor tissue, human hysteromyoma tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Immunogen Information
Full name: actin, alpha 2, smooth muscle, aorta
Calculated molecular weight: 377 aa, 42 kDa
Observed molecular weight: 43 kDa
Gene ID (NCBI):
Gene symbol ACTA2
Synonyms a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, Actin, aortic smooth muscle Actin, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, sma, α SMA, α-sma Background ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins. 59
WB : 1:1000-1:8000 IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB IHC : 1:1500-1:6000 IF : 1:20-1:200
杭州浩克生物
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