mouse brain tissue were subjected to SDS PAGE followed by western blot withHK210001 (ABL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. IP Result of anti-ABL2 (IP:HK210001， 4ug; Detection:HK210001 1:500) with HeLa cells lysate 1200ug. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide usingHK210001 (ABL2 Antibody) at dilution of 1:50 (under 10x lens). Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using HK210001 (ABL2 Antibody) at dilution of 1:50 (under 40x lens).
Species Specificity: human, mouse, rat
Cited Species: human, mouse
Positive WB detected in: mouse brain tissue
Positive IP detected in: HeLa cells
Positive IHC detected in: human liver cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Immunogen Information
Synonyms Abelson related gene protein, ABL2, ABLL, ARG, Tyrosine protein kinase ABL2, Tyrosine protein kinase ARG Background ABL2(Abelson tyrosine-protein kinase 2) is also named as ABLL, ARG and belongs to the protein kinase superfamily. It is a membrane-anchored nonreceptor tyrosine kinase that is a novel mediator of SEMA3F-induced RhoA inactivation and collapsing activity and it has a role in cytoskeletal rearrangements by its C-terminal F-actin- and microtubule-binding sequences. ABL2 can be polyubiquitinated and the polyubiquitination of ABL2 leads to degradation. This protein has 9 isoforms produced by alternative splicing with the molecular weight from 114 kDa to 128 kDa. The antibody is specific to the others except the 60 kDa isoform of ABL2.
WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB IHC : 1:20-1:200
杭州浩克生物
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