MSH6兔单克隆抗体

价格￥600
规格100ul
货号HKZ150126
单位支
品牌HaoKebio

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品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HKZ150126	MSH6兔单克隆抗体	100ul	支	600

MSH6
Recombinant Rabbit Monoclonal Antibody Product Datasheet

Catalog# BX50003 Clone# BP6007

Predicted Molecular Wt:
153kDa
Purity:   ProA affinity purified IgG
Species Cross-reactivity:
Human
Form:   Liquid
Applications:
IHC-P
Swissprot ID:   P52701

Background:
MSH6 is a mismatch repair protein which is deficient in a high proportion of patients with microsatellite instability (MSI-H). It has been suggested that the deficiencies in DNA mismatch repair protein(s) can be seen in some malignancies such as hereditary
nonpolyposis colorectal cancer (HNPCC) and endometrial cancer.

MSH6 expressed in all proliferating cells participate in repair of base-base mismatch, that occur during DNA replication. Loss of
MSH6 expression leads to an accumulation of DNA replication errors in the proliferating cells, particularly in areas of the genome with short repetitive nucleotide sequences, a phenomenon known as microsatellite instability (MSI).

MSH6 always used as panel with MLH1, MSH2, PMS2, and may be useful to aid in identifying the most probable gene responsible for the MSI.

Subcellular location:
Nucleus
Recommended method:
Heat induced epitope retrieval with Tris-EDTA buffer   (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
Immunogen:
Synthetic peptide corresponding to MSH6 residues     within aa1‐100 of MSH6 was used as an immunogen.
Storage Buffer:
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.
Storage conditions:
-25°C to -18°C
Storage instructions:
Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles.
Recommended Dilutions:
IHC-P:     1:100-1:200

Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded
sections) analysis of human colon tissue labelling MSH6 with BP6007. Heat
mediated antigen retrieval was performed using
Tris/EDTA buffer pH 9.0
Background References:
1. Blackwell LJ., et al. J Biol Chem. 1998 Nov 27;273(48):32055-62.