Vimentin兔单克隆抗体

价格￥600
规格100ul
货号HKZ150129
单位支
品牌HaoKebio

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品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HKZ150129	Vimentin兔单克隆抗体	100ul	支	600

Vimentin
Recombinant Rabbit Monoclonal Antibody Product Datasheet

Catalog# BX50006 Clone# BP6010

Predicted Molecular Wt:
54kDa
Purity:   ProA affinity purified IgG
Species Cross-reactivity:
Human
Form:   Liquid
Applications:
IHC-P
Swissprot ID:   P08670
Background:
Vimentin is the most common member of intermediate filament (IF) family and one of the main components in cytoskeleton
structure. It is essential in the role of cell integrity and cytoskeletal stability. The reorganization of vimentin, similar to all IF
proteins, occurs during different stages of the cell cycle and cell signaling by a site-specific phosphorylation (serine and threonine residues).

Vimentin is expressed in a wide variety of mesenchymal cell types: fibroblasts, endothelial cells etc., and in a number of other cell
types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. However, in non-vascular smooth muscle cells, vimentin is often replaced by desmin. In striated muscle, vimetin is also replaced by desmin. However, during regeneration,    vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin,
sometimes in scarce amounts. In tumor tissues, it is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells.

In combination with a panel of antibodies, it is used to identify tumor with mesenchymal origin and malignant melanoma. Additionally, vimentin is a useful control marker for proper tissue processing.

Subcellular location:
Cytoplasm
Recommended method:
Heat induced epitope retrieval with Tris-EDTA buffer   (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
Immunogen:
Synthetic peptide corresponding to Vimentin residues
within aa366‐466 of Vimentin was used as an immunogen.
Storage Buffer:
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.
Storage conditions:
-25°C to -18°C
Storage instructions:
Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles.
Recommended Dilutions:
IHC-P:     1:100-1:200
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections） analysis of human colon
tissue labelling vimentin
with BP6010. Heat mediated antigen retrieval was
performed using Tris/EDTA buffer pH 9.0
Background References: