Topoisomerase II-α兔单克隆抗体

价格￥1000
规格100ul
货号HKZ150134
单位支
品牌HaoKebio

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品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HKZ150134	Topoisomerase II-α兔单克隆抗体	100ul	支	1000

Topoisomerase II-α
Recombinant Rabbit Monoclonal Antibody Product Datasheet

Catalog# BX50011 Clone# BP6016

Predicted Molecular Wt:
174kDa
Purity:   ProA affinity purified IgG
Species Cross-reactivity:
Human
Form:   Liquid
Applications:
IHC-P
Swissprot ID:   P11388

Background:
The topoisomerase II-α isoformisa 170 kDa nuclear protein and plays an important role in DNA synthesis and RNA transcription, as well as chromosomal segregation during mitosis.

Topoisomerase II-α is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells, and has been shown to be over-expressed in many human cancers.

Decreased expression of Topoisomerase II-α is the predominant mechanism of resistance to several chemotherapeutic agents.

Subcellular location:
Nucleus
Recommended method:
Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
Immunogen:
Synthetic peptide corresponding to Topo II α residues
within aa1431-1531 of Topo II α was used as an immunogen.
Storage Buffer:
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.
Storage conditions:
-25°C to -18°C
Storage instructions:
Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles.
Recommended Dilutions:
IHC-P: 1:100-1:200
Background References:
1. Lynch BJ, et al, Hum Pathol. 1997 Oct;28(10):1180-8.
2. Gupta D, et al,Appl Immunohistochem Mol Morphol.
2001 Sep;9(3):215-21.