杭州浩克生物
Parvalbumin alpha兔单克隆抗体
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| 品牌 | 商品编码 | 商品名称 | 规格 | 单位 | 单价 |
|---|---|---|---|---|---|
| HaoKebio | HKZ150138 | HER2兔单克隆抗体 | 100ul | 支 | 1000 |
Recombinant Rabbit Monoclonal Antibody Product Datasheet
Catalog# BX50015 Clone# BP6020
Predicted Molecular Wt:
138kDa
Purity: ProA affinity purified IgG
Species Cross-reactivity:
Human
Form: Liquid
Applications:
IHC-P
Swissprot ID: P04626
Background:
HER-2 (also called HER-2/neu, c-erbB2, ERBB2 or neu) is a transmembrane receptor tyrosine kinase, which is a proto- oncogene, i.e. its activation causes malignant transformation and increases the malignant potential (cell proliferation,
invasiveness etc.) of the cells. In human cancers HER-2 is activated via gene amplification, which is a genomic mutation where a small fragment at chromosome band 17q12-q21 is multiplied in a cell up to 50-100 folds.
HER-2 is over-expressed in 15-25% of primary breast cancers, can also be found in intestinal type gastric and gastroesophageal carcinomas, ovarian carcinomas, high grade endometrial carcinomas and some salivary duct tumors. Low-level copy number increases have been found also in rare cases of lung tumors.
Studies have shown that c-erbB-2 positive breast cancer usually correlates with negative staining for estrogen and progesterone receptors; thus a poorer predictive outcome is correlated with positive c-erbB-2 staining.
Subcellular location:
Membrane
Recommended method:
Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
Immunogen:
Synthetic peptide according to the aa1155-1255 (C- terminal) of HER-2 was used as an immunogen.
Storage Buffer:
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.
Storage conditions:
-25°C to -18°C
Storage instructions:
Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles.
Recommended Dilutions:
IHC-P: 1:100-1:200
Immunohistochemistry
(Formalin/PFA-fixed
paraffin-embedded
sections) analysis of
human breast cancer
tissue labelling HER-2 with BP6020. Heat mediated
antigen retrieval was performed using
Tris/EDTA buffer pH 9.0
Background References:
1. Olayioye M.A., et.al. Chem. 274:17209-17218(1999).
2. Wang S.C., et.al. Cancer Cell 6:251-261(2004).
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