Estrogen Receptor – α兔单克隆抗体

价格￥1400
规格100ul
货号HKZ150144
单位支
品牌HaoKebio

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品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HKZ150144	Estrogen Receptor – α兔单克隆抗体	100ul	支	1400

Estrogen Receptor (ER) α
Recombinant Rabbit Monoclonal Antibody Product Datasheet

Catalog# BX50021 Clone# BP6026

Predicted Molecular Wt:
66kDa
Purity:   ProA affinity purified IgG
Species Cross-reactivity:
Human
Form:   Liquid
Applications:
IHC-P
Swissprot ID:   P03372

Background:
Estrogen receptor (ER) belongs to the steroid receptor superfamily of nuclear receptors. It is a protein with 553 amino acids. The receptor molecule has three domains, i.e. the central DNA-binding domain, the hormone-binding domain at the C-
terminal, and the transcription-activating domain at the N-terminal. ER mediates regulatory functions of female sex steroids,   mainly 17 (E2), on growth, differentiation and function in several target tissues, including female and male reproductive tract, mammary gland, and skeletal and cardiovascular systems.

Studies have shown ER α is present in the nuclei of epithelial cells in normal breast and endometrial tissues, as well as a subset of breast carcinomas.
Studies with immunohistochemical assay show that positive steroid hormone status has predicted favourable overall, survival, independently of hormonal treatment. Secondly, ER α can be used as a tumour marker, preferentially in combination with an   antibody to progesterone receptor,e.g., in the classification of adenocarcinomas.
Subcellular location:
Nuclear
Recommended method:
Heat induced epitope retrieval with Tris-EDTA buffer   (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
Immunogen:
Synthetic peptide corresponding to ER alpha residues
within aa495‐595 of ER alpha was used as an immunogen.
Storage Buffer:
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.
Storage conditions:
-25°C to -18°C
Storage instructions:
Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles.
Recommended Dilutions:
IHC-P:     1:100-1:200
Background References:
1. Stein B, et.al, Mol Cell Biol. 1995 Sep;15(9):4971-9.
2. Saville B, et.al,J Biol Chem. 2000 Feb 25;275(8):5379- 87.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded
sections) analysis of
human breast cancer
tissue labelling ER α with BP6026. Heat mediated   antigen retrieval was
performed using
Tris/EDTA buffer pH 9.0

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