DOG1兔单克隆抗体

价格￥1000
规格100ul
货号HKZ150150
单位支
品牌HaoKebio

产品介绍

产品参数

资料下载

品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HKZ150150	DOG1兔单克隆抗体	100ul	支	1000

DOG1
Recombinant Rabbit Monoclonal Antibody Product Datasheet
Catalog# BX50027 Clone# BP6032
Predicted Molecular Wt:
114kDa
Purity:   ProA affinity purified IgG
Species Cross-reactivity:
Human
Form:   Liquid
Applications:
IHC-P
Swissprot ID:  Q5XXA6
Background:
DOG1 is a calcium-dependent chloride channel protein that is encoded by a gene called TMEM16A (TMEM16 FLJ10261, ANO1,  ORAOV2, and AOS2) located on chromosome 11q13. DOG1 has many significant functions such as regulation of the cholinergic activity of gastrointestinal smooth muscle and regulation of both the survival and proliferation of cells.
DOG1 is detected in gastrointestinal Cajal cells, acinic cells in salivary glands (apical membraneous staining, particularly in
serous cells), pancreatic centroacinar cells, liver cells, and epithelium of biliary tract, breast, stomach, and prostate. More than 90% of all gastrointestinal stromal tumors (GISTs) are DOG1 positive, irrespective of kit mutation and CD117 positivity. The
staining pattern varies from cytoplasmic to membranous, with usually strong, diffuse intensity.
DOG1 is an important marker in the identification of GIST together with CD117, slightly more sensitive (particularly in gastric
GIST without c-kit mutation) and also more specific than CD117. DOG1 is also useful in the classification of salivary carcinomas, and pancreatic and renal tumors.
Subcellular location:
Membrane/Cytoplasm
Recommended method:
Heat induced epitope retrieval with Tris-EDTA buffer   (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
Immunogen:
Synthetic peptide corresponding to DOG1 residues     within aa1-100 of DOG1 was used as an immunogen.
Storage Buffer:
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.
Storage conditions:
-25°C to -18°C
Storage instructions:
Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles.
Recommended Dilutions:
IHC-P:     1:100-1:200
Background References:
Immunohistochemistry
(Formalin/PFA-fixed
paraffin-embedded
sections analysis of GIST     tissue labelling DOG1 with BP6032. Heat mediated
antigen retrieval was performed using
Tris/EDTA buffer pH 9.0
1. Jung SH, et.al, Gut Liver. 2011 Jun;5(2):171-80.
2. Bergmann F, et.al, Hum Pathol. 2011 Jun;42(6):817-23.

HKZ150150_BP6032_DOG1 下载

暂无