Predicted Molecular Wt: 23kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P07766 Background: Cluster of differentiation 3 (CD3) is a multimeric protein complex, known historically as the T3 complex, and is composed of four distinct polypeptide chains; epsilon (ε), gamma (γ), delta (δ) and zeta (ζ), that assemble and function as three pairs of dimers (εγ, εδ, ζζ). The CD3 complex serves as a T cell co-receptor that associates noncovalently with the T cell receptor (TCR).
CD3 antigen appearance at all stagesofT cell development, and makes it anideal T cell marker for both the detection of normal T cells and T cell neoplasms (lymphomas and leukemias). Subcellular location: Membrane Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide corresponding to CD3E residues within aa100-200 of CD3E was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Smith-Garvin JE, et.al, Annu Rev Immunol. 2009;27:591-619. 2. SalvadoriS, et.al, J Immunol. 1994 Dec 1;153(11):5176- 82. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections analysis of tonsil tissue labelling CD3 with BP6039. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
杭州浩克生物
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