Predicted Molecular Wt: 25kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P11912 Background: Cluster of differentiation CD79a also known as B-cell antigen receptor complex-associated protein alpha chain is a protein that in humans is encoded by the CD79A gene. The CD79a protein together with the related CD79b protein, forms a dimer associated with membrane-bound immunoglobulin in B-cells, thus forming the B-cell antigen receptor (BCR),and plays multiple and diverse roles in B cell development and function.
CD79a protein is expressed in virtually all mature and immature B cells and the majority of B-cell neoplasms, including precursor B–acute lymphocytic leukemia (pre-B–ALL), L and H lymphocyte predominance Hodgkin’s lymphoma, and many plasma cell tumors.
Therefore, antibodies to CD79a are useful in the differential diagnosis of B-cell neoplasms from T-cell neoplasms or myeloid neoplasms, or L and H lymphocyte predominance Hodgkin’s lymphoma from classic Hodgkin’s lymphoma. In addition, CD79a is a useful marker in the diagnosis of precursor B–acute lymphoblastic leukemia (pre-B–ALL) because many of these tumors are negative for other B-cell markers, such as CD20 and CD45RA. Subcellular location: Membrane Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide corresponding to CD79a residues within aa1-100 of CD79a was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Seda V, et.al, Eur J Haematol. 2015 Mar;94(3):193-205. 2. Chu PG, et.al,Appl Immunohistochem Mol Morphol. 2001 Jun;9(2):97-106. Immunohistochemistry (Formalin/PFA-fixedparaffin-embedded sections analysis of tonsil tissue labelling CD79a with BP6040. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
您好!请登录