Predicted Molecular Wt: 45kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: Q02962 Background: PAX-2 is a member of the PAX family of transcription factors that, together with PAX-8, is involved in the regulation of the organogenesis of the kidney and the Müllerian system. Among the non-neoplastic tissue, PAX-2 was expressed in glomerular parietal epithelial cells, renal collecting duct cells, atrophic renal tubular cells, epithelial cells of ovarian surface, fallopian tube, endocervix, endometrium. Among the primary neoplasms, PAX-2 was mainly noted in renal cell carcinoma, carcinomas of Müllerian origin, nephrogenic adenomas. Recent investigations have indicated that PAX-2 could serve as a useful immunohistochemical marker that can assist in the diagnosis of epithelial tumors of the kidney, as well as some gynecologic (Müllerian) neoplasms. Subcellular location: Nucleus Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide corresponding to PAX-2 residues within aa300-400 of PAX-2 was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Zhai QJ, et.al,Appl Immunohistochem Mol Morphol. 2010 Jul;18(4):323-32. 2. Ordóñez NG, et.al, Adv Anat Pathol. 2012 Nov;19(6):401-9. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections analysis of kidney tissue labelling PAX-2 with BP6044. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
杭州浩克生物
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