CD19 Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50041 Clone# BP6046 Predicted Molecular Wt: 61kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P15391 Background: The human CD19 antigen is a transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily, which initiates a series of biological responses that are likely to be of central importance to B-cell signaling and/or growth regulation and the development of a humoral immune response. CD19 is specifically expressed in normal and neoplastic B cells, as well as follicular dendritic cells. In normal tissues, it is expressed from pre-B cells until the terminal differentiation to plasma cells. And in tumors, its expression is highly conserved on most B cell tumors. It is expressed in most acute lymphoblastic leukemias (ALL), chronic lymphocytic leukemias (CLL) and B cell lymphomas. CD19 is one of the most reliable surface biomarker for B cells and the CD19 monoclonal antibodies have been explored for lymphoma therapy. Subcellular location: Membrane Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide corresponding to CD19 residues within aa456-556 of CD19 was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Wang K, et.al, Exp Hematol Oncol. 2012 Nov 29;1(1):36. 2. Hammer O,et.al, MAbs. 2012 Sep-Oct;4(5):571-7. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections analysis of tonsil tissue labelling CD19 with BP6046. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
杭州浩克生物
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