E-Cadherin Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50043 Clone# BP6048 Predicted Molecular Wt: 97kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P12830 Background: E-cadherin is a transmembrane, calcium dependent cell adhesion protein that mediates cell to cell adhesion and maintains structural and functional integrity of epithelial tissues. It also has pivotal barrier functions and maintains the polarity of epithelial cells. Reduced or aberrant E-cadherin expression breaks cell to cell contacts, and thus, cells acquire the ability to migrate. In normal tissues, immunostaining of E-cadherin is localized to the membrane of epithelial cells, consistent with its role in cell adhesion. And in tumor tissues, E-cadherin stains positively in glandular epithelium as well as adenocarcinomas of the lung, gastrointestinal tract, and ovary. It has also been shown to be positive in some thyroid carcinomas. A combination of E-cadherin and p120 catenin may help distinguish ductal carcinoma of the breast from lobular carcinoma. And also, several studies have reported that reduced E-cadherin expression is correlated with poor prognosis in several types of carcinomas. Subcellular location: Membrane Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide corresponding to E-Cadherin residues within aa600-700 of E-cadherin was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Xie Y, et al. Oncotarget. 2017 Aug 4;8(37):62489-62499. 2. F. van Roy, et al. Cell Mol Life Sci. 2008 Nov;65(23):3756-88. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections analysis of liver tissue labelling E- Cadherin with BP6048. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
杭州浩克生物
您好！请登录