Cytokeratin 14兔单克隆抗体

Cytokeratin 14克隆抗体

  • 价格¥600
  • 规格100ul
  • 货号HKZ150047
  • 单位
  • 品牌HaoKebio

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HaoKebioHKZ150047Cytokeratin 14兔单克隆抗体100ul600
Cytokeratin 14
Recombinant Rabbit Monoclonal Antibody Product Datasheet
Catalog#  HKZ150047 Clone#  BP6052
Predicted Molecular Wt:
52kDa
Purity:   ProA affinity purified IgG
Species Cross-reactivity:
Human
Form:   Liquid
Applications:
IHC-P
Swissprot ID:   P02533
Background:
Cytokeratin 14 is a member of the type I keratin family of intermediate filament proteins. It always pairs with the type II
keratin K5 and form the primary keratin pair of the keratinocytes of stratified squamous epithelia, including the epidermis as well as mucosal non-keratinizing stratified squamous epithelia.
Cytokeratin 14 is strongly expressed in the undifferentiated basal cell layer containing the stem cells and are down-regulated  in the differentiating suprabasal cell layers. Otherwise, in the widely well stratified follicular outer rootsheath, cytokeratin 14 is uniformly expressed throughout all layers. The expression spectrum of cytokeratin 14 in tumors corresponds well to the
patterns in normal epithelia. Thus, most squamous cell carcinomas as well as malignant mesotheliomas strongly express this keratin whereas little, focal, or no expression is found in adenocarcinomas.
Cytokeratin 14 may be a useful marker in the differential diagnosis of squamous cell carcinoma from other epithelial tumors. Recent studies also indicate that CK14 expression in breast cancer corresponded with poor clinical outcome and that CK14
may have diagnostic value in the sub-classification of NSCLC.
Subcellular location:
Cytoplasm
Recommended method:
Heat induced epitope retrieval with Tris-EDTA buffer   (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
Immunogen:
Synthetic peptide corresponding to cytokeratin 14
residues within aa372-472 of cytokeratin 14 was used as an immunogen.
Storage Buffer:
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.
Storage conditions:
-25°C to -18°C
Storage instructions:
Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles.
Recommended Dilutions:
IHC-P:     1:100-1:200
Background References:
1.  Reis-Filho JS, et.al. Virchows Arch. 2003 Aug;443(2):122-32.
2.  Moll R, et.al. Histochem Cell Biol. 2008 Jun;129(6):705- 33.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded
sections analysis of
esophagus tissue
labelling cytokeratin 14 with BP6052. Heat
mediated antigen
retrieval was performed using Tris/EDTA buffer    pH 9.0

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