Cytokeratin 18兔单克隆抗体

价格￥600
规格100ul
货号HKZ150172
单位支
品牌HaoKebio

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品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HKZ150172	Cytokeratin 18兔单克隆抗体	100ul	支	600

Cytoketatin 18
Recombinant Rabbit Monoclonal Antibody Product Datasheet
Catalog#BX50049 Clone# BP6054
Predicted Molecular Wt:
48kDa
Purity:   ProA affinity purified IgG
Species Cross-reactivity:
Human
Form:   Liquid
Applications:
IHC-P
Swissprot ID:   P05783
Background:
Cytokeratin 18 (also named keratin 18) is a type Icytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family.
In some epithelial cell types, keratin 8 and keratin 18 are the sole keratins present. The classical example is the liver, with keratin 8/keratin 18 representing the characteristic and only keratin pair of normal hepatocytes. The same is true for other highly
specialized parenchymatous epithelia such asacinar cells of the pancreas, proximal tubular epithelial cells of the kidney, and
certain endocrine cells such as pancreatic islet cells. Furthermore, keratin 8/keratin 18 occur together with other keratins—in various pseudostratified (e.g. respiratory) and complex (e.g. glandular) epithelia and in the urothelium; in these composite
epithelial tissues, keratin 8 and keratin18 are often most prominent in the lumen-lining cells. Even in non-keratinizing stratified squamous epithelia, keratin 8 and keratin 18 may be focally expressed in the basal cell layer, together with keratin 19 and the   constitutive stratified-epithelial keratins.
In regard to malignant tumors, keratin 8 and keratin 18 are expressed in most carcinomas except for some differentiated
squamous cell carcinomas. Therefore, keratin 8 and keratin 18 antibody strongly stain most adenocarcinomas, hepatocellular carcinomas, renal cell carcinomas, and neuroendocrine carcinomas.
Subcellular location:
Cytoplasm
Recommended method:
Heat induced epitope retrieval with Tris-EDTA buffer   (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
Immunogen:
Synthetic peptide corresponding to cytokeratin 18
residues within aa330-430 of cytoketatin 18 was used as an immunogen.
Storage Buffer:
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.
Storage conditions:
-25°C to -18°C
Storage instructions:
Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles.
Recommended Dilutions:
IHC-P:     1:100-1:200
Background References:
1. Wiedenmann B, et.al. Proc Natl Acad Sci U S A. 1986 May;83(10):3500-4.
2. Quinn B. Am J Surg Pathol. 1998 May;22(5):550-6.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded
sections analysis of liver tissue labelling
cytokeratin 18 with
BP6054. Heat mediated antigen retrieval was
performed using
Tris/EDTA buffer pH 9.0

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