Type II Cytokeratins Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50053 Clone# BP6058 Predicted Molecular Wt: 66/65/64/57/62/60/51/54kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Background: The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK1, CK2, CK3, CK4, CK5, CK6, CK7 and CK8. It is usually used in a cytokeratin cocktail with type Icytokeratins antibody. Subcellular location:Cytoplasm Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide corresponding to Type II Cytokeratins residues within aa300-400 of Type II Cytokeratins was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Spagnolo DV, et al. Am J Clin Pathol. 1985 Dec;84(6):697-704. 2. Eichner R, et al. J Cell Biol. 1984 Apr;98(4):1388-96. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections analysis of liver tissue labelling Type II Cytokeratins with BP6058. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
杭州浩克生物
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