Type II Cytokeratins兔单克隆抗体

价格￥600
规格100ul
货号HKZ150176
单位支
品牌HaoKebio

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品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HKZ150176	Type II Cytokeratins兔单克隆抗体	100ul	支	600

Type II Cytokeratins
Recombinant Rabbit Monoclonal Antibody Product Datasheet
Catalog# BX50053 Clone# BP6058
Predicted Molecular Wt:
66/65/64/57/62/60/51/54kDa
Purity:   ProA affinity purified IgG
Species Cross-reactivity:
Human
Form:   Liquid
Applications:
IHC-P
Background:
The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist.   They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation.
This antibody can detect high molecular weight CK1, CK2, CK3, CK4, CK5, CK6, CK7 and CK8. It is usually used in a cytokeratin cocktail with type Icytokeratins antibody.
Subcellular location:Cytoplasm
Recommended method:
Heat induced epitope retrieval with Tris-EDTA buffer   (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
Immunogen:
Synthetic peptide corresponding to Type II Cytokeratins residues within aa300-400 of Type II Cytokeratins was used as an immunogen.
Storage Buffer:
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.
Storage conditions:
-25°C to -18°C
Storage instructions:
Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles.
Recommended Dilutions:
IHC-P:     1:100-1:200
Background References:
1. Spagnolo DV, et al. Am J Clin Pathol. 1985 Dec;84(6):697-704.
2. Eichner R, et al. J Cell Biol. 1984 Apr;98(4):1388-96.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded
sections analysis of liver tissue labelling Type II     Cytokeratins with
BP6058. Heat mediated antigen retrieval was
performed using
Tris/EDTA buffer pH 9.0

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