WT1兔单克隆抗体

价格￥1000
规格100ul
货号HKZ150180
单位支
品牌HaoKebio

产品介绍

产品参数

资料下载

品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HKZ150180	WT1兔单克隆抗体	100ul	支	1000

WT1
Recombinant Rabbit Monoclonal Antibody Product Datasheet
Catalog# BX50057 Clone# BP6062
Predicted Molecular Wt:
49kDa
Purity:   ProA affinity purified IgG
Species Cross-reactivity:
Human
Form:   Liquid
Applications:
IHC-P
Swissprot ID:   P19544
Background:
The WT1 gene located at chromosome 11p13 codes for a transcription factor,a DNA-binding nucleoprotein, that plays a role primarily in the development of genitourinary organs. There are at least eight isoforms ranging between 52 and 62 kDa
produced by a combination of alternative splicing and RNA editing. WT1 is synthesized and reside in the cytoplasm in an
inactive form. When activated through phosphorylation it is translocated to the nucleus. WT1 influences cell proliferation by suppressing bcl-2 and regulating cadherin and p53.
In normal epithelia, nuclear WT1 expression is largely restricted to ovary (surface epithelium and inclusion cysts) and fallopian tube, while WT1 is not found in endometrial or cervical epithelium. As regards nonepithelial cells, nuclear WT1 is found in
mesothelium and some submesothelial stromal cells, stromal cells of the female genital tract, testicular non-germinal cells,    and kidney (podocytes). In tumor tissues, WT1 is detected in tumor cells of Wilms’ Tumor (also  known as nephroblastoma)    and mesothelioma. Additionally, WT1 expression has been found in ovarian serous carcinomas and some breast carcinomas.
WT1 is particularly used for distinguishing malignant mesothelioma and ovarian serous carcinoma from nonserous carcinomas. As for malignant mesothelioma, calretinin and WT1 are superior to cytokeratin 5/6, N-cadherin and
thrombomodulin. WT1 is also applicable for the differential diagnostic of small cell childhood tumours.
Subcellular location:
Nucleus
Recommended method:
Heat induced epitope retrieval with Tris-EDTA buffer   (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
Immunogen:
Synthetic peptide corresponding to WT1 residues
within aa1-100 of  WT1 was used as an immunogen.
Storage Buffer:
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.
Storage conditions:
-25°C to -18°C
Storage instructions:
Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles.
Recommended Dilutions:
IHC-P:     1:100-1:200
Background References:
1. Carpentieri DF etal. Mod Pathol. 2002 Oct;15(10):1080-6.
2. Goldstein NS etal. Am J Clin Pathol. 2002 Apr;117(4):541-5.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded
sections) analysis of
ovarian cancer labelling WT1 with BP6062. Heat mediated antigen
retrieval was performed using Tris/EDTA buffer    pH 9.0.

HKZ150180_BP6062_WT1 下载

暂无