Cytokeratin 6 Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# HKZ150060 Clone# BP6065 Predicted Molecular Wt: 60kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P48668 Background: Cytokeratin 6 is an epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. This protein always paired with the type-I keratin K16. Cytokeratin 6 have been identified in epidermis only in samples from plantar glabrous skin while in hairy skin this keratin appeared to be absent in interfollicular epidermis but were clearly present in hair follicle outer rootsheath, and this protein also consistently expressed in non-keratinizing stratified squamous epithelia. Among tumors, cytokeratin 6 is typically and strongly expressed in squamous cell carcinomas of different sites, preferentially in inner, maturing layers of the tumor cell nests. Cytokeratin 6 is always used with cytokeratin 5 and is a useful aid for classification of epithelioid mesotheliomas. Subcellular location: Cytoplasm Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide corresponding to Cytokeratin 6 residues within aa464-564 of Cytokeratin 6 was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. R Mollet al. Histochem Cell Biol (2008) 129:705–733. 2. R Mollet al. Cell, Vol. 31, 1 l-24, November 1982. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections analysis of esophagus cancer tissue labelling Cytokeratin 6 with BP6065. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
杭州浩克生物
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