p21 Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50063 Clone# BP6068 Predicted Molecular Wt: 18kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P38936 Background: p21, also known as cyclin-dependent kinase inhibitor 1 or CDK-interacting protein 1, is a protein that in humans is encoded by the CDKN1A gene located on chromosome 6 (6p21.2). The p21 protein binds to and inhibits the activity of cyclin-CDK2 or – CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. p21 is expressed in all adult human tissues. In tumors, the expression of p21 has been studied by immunohistochemical methods in a wide range of human tumors, such as gastric carcinoma, non-small cell lung carcinoma, and thyroid carcinoma. The expression of p21 is associated with favorable prognosis in various tumors. Subcellular location: Nucleus Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide corresponding to p21 residues within aa1-100 of p21 was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Komiya T etal. Clin Cancer Res. 1997 Oct;3(10):1831-5. 2. Natsugoe S et al. Clin Cancer Res. 1999 Sep;5(9):2445- 9. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections analysis of cervix cancer tissue labelling p21 with BP6068. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
杭州浩克生物
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