CD45 Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50068 Clone# BP6073 Predicted Molecular Wt: 147kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P08575 Background: CD45 is a family of single chain transmembrane glycoproteins, consisting of at least four isoforms which share a common large intracellular domain. Their extracellular domains are rod shaped, heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1q31-32. CD45 is exclusively expressed in haematolymphoid cells. Almost all haematolymphoid cells, including precursor cells and mature B- and T-lymphocytes, granulocytes, monocytes/histiocytes and interdigitating reticulum cells and follicular dendritic cells express CD45. This protein is detected in the large majority of haematolymphoid neoplasms, i.e., leukemias and malignant lymphomas. Overall, about 90% of malignant lymphomas are CD45 positive. CD45 is an important marker in the primary tumor screening panel in order to identify haematolymphoid differentiation. Loss of CD45 in precursor B-cell neoplasms is a negative prognostic parameter. Subcellular location:Membrane Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Recombinant fragment within Human CD45 aa 1 to aa600. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Dahlke MH etal. Leuk Lymphoma. 2004 Feb;45(2):229- 36. 2. Gaertner EM etal. Am J Clin Pathol. 2001 Apr;115(4):589-97. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections analysis of tonsil tissue labelling CD45 with BP6073. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
杭州浩克生物
您好！请登录