p120兔单克隆抗体

价格￥1000
规格100ul
货号HKZ150193
单位支
品牌HaoKebio

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品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HKZ150193	p120兔单克隆抗体	100ul	支	1000

p120
Recombinant Rabbit Monoclonal Antibody Product Datasheet
Catalog# BX50070 Clone# BP6075
Predicted Molecular Wt:
108kDa
Purity:   ProA affinity purified IgG
Species Cross-reactivity:
Human
Form:   Liquid
Applications:
IHC-P
Swissprot ID:  O60716
Background:
Alpha-catenin and beta-catenin bind to the intracellular domain of E-cadherin while p120 catenin binds E-cadherin at a juxta- membrane site. The complex stabilizes tight junctions. In the cell, p120 catenin localized to the E-cadherin/catenins cell
adhesion complex, directly associates with cytoplasmic C-terminus of E-cadherin and may similarly interact with other cadherins.
p120 is a proliferation-associated nucleolar protein found in most human malignant tumors, but not in resting normal cells. In colorectal cancer the altered localization of p120 catenin corresponds with loss of cytoplasmic localization of E-cadherin.
Studies have shown accurate categorization of ductalvs. lobular neoplasia in the breast was achieved with p120 staining. p120 expression further clarifies the separation of low-grade ductal carcinoma in situ from lobular neoplasia. Studies also have shown that altered expression of p120 catenin antibody predicts poor outcome in invasive breast cancer.
Subcellular location:
Cytoplasm and membrane
Recommended method:
Heat induced epitope retrieval with Tris-EDTA buffer   (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
Immunogen:
Synthetic peptide corresponding to p120 residues within aa900-1000 of  p120 was used as an
immunogen.
Storage Buffer:
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.
Storage conditions:
-25°C to -18°C
Storage instructions:
Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles.
Recommended Dilutions:
IHC-P:     1:100-1:200
Background References:
1. Talvinen K, et al. Altered expression of p120 catenin predicts poor outcome in invasive breast cancer. J     Cancer Res Clin Oncol. 2010 Sep; 136(9):1377-87.
2. Chivukula M, et al. Pleomorphic lobular carcinoma in
situ (PLCIS) on breast core needle biopsies: clinical
significance and immunoprofile. Am J Surg Pathol. 2008 Nov; 32(11):1721-6.
Immunohistochemistry
(Formalin/PFA-fixed
paraffin-embedded
sections analysis of colon tissue labelling p120 with BP6075. Heat mediated    antigen retrieval was
performed using
Tris/EDTA buffer pH 9.0

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