Cyclin D1兔单克隆抗体

价格￥1000
规格100ul
货号HKZ150071
单位支
品牌HaoKebio

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品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HKZ150071	Cyclin D1兔单克隆抗体	100ul	支	1000

Cyclin D1
Recombinant Rabbit Monoclonal Antibody Product Datasheet
Catalog# HKZ150071 Clone# BP6076
Predicted Molecular Wt:
34kDa
Purity:   ProA affinity purified IgG
Species Cross-reactivity:
Human
Form:   Liquid
Applications:
IHC-P
Swissprot ID:   P24385
Background:
Cyclin D1 (synonymes: PRAD1, parathyroid adenomatosis 1,CCND1) is a 295 amino acid protein, 36 kDa, which belongs to the
highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is
required for cell cycle G1/S transition. The protein has been shown to interact with tumour suppressor protein Rb andits expression is regulated positively by Rb.
The expression is cell cycle dependant, maximal in G1 and minimal in S phase. Localization is mainly nuclear. In normal tissues, Cyclin D1 expression is restricted to the proliferative zone of epithelial tissues, endothelium and some fibroblasts. There is no   expression in lymphoid tissue.
Mutations with amplification of the cyclin D1 gene, with overexpression of the protein, which alters cell cycle progression, are observed frequently in a variety of tumours and may contribute to tumourigenesis.
In diagnostic pathology, immunohistochemical detection of Cyclin D1 is used mainly for the diagnosis of mantle cell
lymphoma. While other methods of cyclin D1 detection are being explored, immunohistochemistry is still widely used and anti-cyclin D1 antibodies remain an essential component in a small B-cell lymphoproliferative disorder panel.
Subcellular location:
Nucleus
Recommended method:
Heat induced epitope retrieval with Tris-EDTA buffer   (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
Immunogen:
Synthetic peptide corresponding to residues within   aa200-295 of  Cyclin D1 was used as an immunogen.
Storage Buffer:
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.
Storage conditions:
-25°C to -18°C
Storage instructions:
Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles.
Recommended Dilutions:
IHC-P:     1:100-1:200
Background References:
1. Hirai, H. et al. Novel INK4 proteins, p19 and p18,are specific inhibitors of the cyclin D-dependent kinases CDK4 and CDK6. Mol Cell Biol. 1995 May; 15(5): 2672–2681.
2. Sherr, C.J. Cancer cell cycles. Science. 1996 Dec 6;274(5293):1672-7.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded
sections analysis of
mantle cell lymphoma    labelling Cyclin D1 with  BP6076. Heat mediated antigen retrieval was
performed using
Tris/EDTA buffer pH 9.0.

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