Cytokeratin 1 Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50072 Clone# BP6077 Predicted Molecular Wt: 66kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P04264 Background: Cytokeratin 1 may regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). Defects in Cytokeratin 1 area cause of bullous congenital ichthyosiformerythroderma (BCIE); also known as epidermolytic hyperkeratosis (EHK) or bullouserythroderma ichthyosiformiscongenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and anichthyoticerythrodermaat birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinouslayer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop. In diagnostic pathology, immunohistochemical detection of cytokeratin 1 is usually used to labelsquamous, ductaland complex epithelia combined with other high molecular weight cytokeratins such as cytokeratins 5, 10 and 14. Resultsaid in the classification of prostatic adenocarcinoma and in the classification of neoplastic tissue as carcinoma of epithelial origin. Subcellular location: Plasma membrane, cytoplasm Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide corresponding to residues withinaa200-300 of Cytokeratin 1 was used as an immunogen. Background References: 1. Schaller G et al. Immunohistochemical detection of keratins 1 and 13 as differentiation markers in the hormone- dependent human vaginal epithelium. Gynecol Obstet Invest. 1990;30(2):94-6. 2.Murakami Y, et al. Immunohistochemical interpretation of early epithelial disorders of pyriform sinus. Ann Otol RhinolLaryngol. 1990 Oct;99(10 Pt 1):782-8. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections analysis of esophagus tissue labelling Cytokeratin 1 with BP6077. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
您好!请登录