hCG Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50075 Clone# BP6080 Predicted Molecular Wt: 13kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P01215 Background: Human chorionic gonadotropin antibody (hCG) is a glycoprotein hormone synthesized in syncytiotrophoblastic cells of placenta and in certain trophoblastic tumors. The hormone-specific alpha chains have molecular weights of 13 kDa. HCG is found in moles and choriocarcinoma, chorionic components of germ cell tumours, and syncytiotrophoblast like cells in seminoma/dysgerminoma and embryonal carcinoma. In diagnostic pathology, hCG is a useful marker for classification of germ cell tumours, identification of extragonadal germ cell tumours. Subcellular location: Cytoplasm Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide corresponding to residues within aa1-100 of hCG was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. LoschA, Kainz C. Acta Obstet Gynecol Scand. 1996 Sep;75(8):753-6. 2. Kido A, Mori M, Adachi Y, Yukaya H, Ishida T, Sugimachi K. Surg Today. 1996;26(12):966-70. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of placenta labelling hCG with BP6080. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
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