CD38 Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# HKZ150077 Clone# BP6082 Predicted Molecular Wt: 34kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P28907 Background: CD38, also known as ADP-ribosyl cyclase, synthesizes the second messagers cyclic ADP-ribose and nicotinate-adenine dinucleotide phosphate, the former a second messenger for glucose-induced insulin secretion. CD38 also has cADPr hydrolase activity. It is found on immature cells of the B and T cell lineages but not on most mature resting peripheral lymphocytes. It is also present on thymocytes, pre-B cells, germinal center B cells, mitogen-activated T cells, Ig-secreting plasma cells, monocytes, NK cells, erythroid and myeloid progenitors in the bone marrow and brain cells. CD38 isuesd to identify plasma cells and plasmablastic differentiation, and evaluate the prognosis for B-cell chronic lymphocytic leukemia (CLL) patients. Subcellular location: Membrane Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide corresponding to residues within aa200-300 of CD38 was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Chini CC et al. Clin Cancer Res 20:120-30 (2014). 2. Kobayashi M etal. World J Gastroenterol 20:3597-608 (2014). Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of tonsil labelling CD38 with BP6082. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
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