Melan-A Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50081 Clone# BP6086 Predicted Molecular Wt: 13kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: Q16655 Background: Melan-A is involved in melanosome biogenesis by ensuring the stability of GPR143 and plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes. Melan-A is specifically expressed in malignant melanoma. Melan-A is one of the most valuable melanoma markers. Melan-A is useful in confirming melanocytic differentiation of primary cutaneous tumours, differentiating these from neurothekeoma and benign fibrous histiocytoma. Subcellular location: Cytoplasm Recommended Method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Full length protein corresponding to Human Melan-A. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage Conditions: -25°C to -18°C Shipment Instructions: Shipped on blue ice. Upon delivery store at -25°C to – 18°C. Avoid freeze / thaw cycles. Recommended Dilution: IHC-P: 1:100-1:200 Background References: 1. Hoashi T., Watabe H., Muller J.,Vieira W.D., Hearing V.J.J. Biol. Chem. 280:14006-14016(2005) 2. Giordano F., Bonetti C., Surace E.M., Marigo V., Raposo G.Hum. Mol. Genet. 18:4530-4545(2009) Immunohistochemistry (Formalin/PFA-fixed paraffin- embedded sections) analysis of malignant melanoma labelling Melan-A with BP6086.
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