Myosin (Smooth Muscle) Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50086 Clone# BP6091 Predicted Molecular Wt: 227kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P35749 Background: Smooth Muscle Myosin, heavy chain (SMM-HC) is a cytoplasmic structural protein that is a major component of the contractile apparatus of the smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early and is specific for smooth muscle development. SM-MHC stains the intact myoepithelial cell (MEC) layers present in benign and in situ malignant breast and bronchioloalveolar lesions and is therefore very helpful in distinguishing between benign and malignant tumors. The antibody reacts with smooth muscle cells and myoepithelial cells, but not with myofibroblasts. It is very helpful in distinguishing between benign sclerosing breast lesions and infiltrating carcinomas in difficult cases since it strongly stains the myoepithelial layer in the benign lesions while it is negative in the infiltrating carcinomas.Subcellular location: Cytoplasm Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide corresponding to Myosin (Smooth Muscle) residues within aa1-100 of Myosin (Smooth Muscle) was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Hilson JB, et al. Am J Surg Pathol. 2010 Jun; 34(6):896-900. 2. Nicolas MM, et al. Hum Pathol. 2010 May; 41(5):663-71 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections analysis of liomyoma tissue labelling Myosin (Smooth Muscle) with BP6090. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
杭州浩克生物
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