Calponin Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50088 Clone# BP6093 Predicted Molecular Wt: 33kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P51911 Background: Calponin a 33 kDa polypeptide, is a cytoskeleton-associated actin-binding protein that also interactstropomyosin and calmodulin. Calponin antibody has been found to be useful as a marker for myoepithelial and basal lamina in differentiating microinvasive from in situ ductal carcinomas of the breast. Calponin antibody may also have applications in malignant myoepithelium and pleomorphic adenoma of salivary gland as well as a useful marker for fine needle aspirates of papillary breast lesions. The antibody has been found in tissue extracts from smooth muscle, but not in fibroblast extracts. Subcellular location: Cytoplasm Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide corresponding to residues on the C terminus of Human Calponin was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Mosunjac MB, et al. Diagn Cytopathol. 2000 Sep; 23(3):151-5. 2. Abdulrahman SS, et al. Saudi Dent J 2019 Jan;31(1):105-114. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections analysis of liomyoma tissue labelling Calponin with BP6093. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
杭州浩克生物
您好！请登录