EGFR（ L858R)兔单克隆抗体

价格¥2200
规格100ul
货号HKZ150216
单位支
品牌HaoKebio

产品介绍

产品参数

资料下载

品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HKZ150216	EGFR（ L858R)兔单克隆抗体	100ul	支	2200

EGFR（ L858R）
Recombinant Rabbit Monoclonal Antibody Product Datasheet
Catalog# BX50093 Clone# BP6098
Predicted Molecular Wt:
134kDa
Purity:   ProA affinity purified IgG
Species Cross-reactivity:
Human
Form:   Liquid
Applications:
IHC-P
Swissprot ID:   P00533
Background:
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha,amphiregulin, epigen/EPGN,
BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or
heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter
proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream
signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and
probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and
increases its interaction with SRC and CTNNB1/beta-catenin. Plays a role in enhancing learning and memory performance (By similarity).
The antibody is specific expressed on EGFR(L858R) mutated lung cancer.
Subcellular location:
Cytomembrane
Recommended method:
Heat induced epitope retrieval with Tris-EDTA buffer  (pH 9.0), primary antibody incubate at 4°C overnight.
Immunogen:
A synthetic peptide corresponding to EGFR residues within aa800-900 of  EGF receptor (mutated L858R) was used as an immunogen.
Storage Buffer:
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.
Storage conditions:
-25°C to -18°C
Storage instructions:
Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles.
Recommended Dilutions:
IHC-P:     1:100-1:200
Background References:
1. Koo JS, et al. Neoplasma. 2011; 58(1):27-34.
2.    Lu X,Yu L, et al.  MedResRev2018;38:1550–1581.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded
sections analysis of
lung carcinoma  tissue (mutated L858R)
labelling EGFR (L858R) with BP6098. Heat
mediated antigen
retrieval was performed using Tris/EDTA buffer    pH 9.0

HKZ150216_BP6098_ EGFR(L858R)下载

暂无