CD2 Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50102 Clone# BP6107 Predicted Molecular Wt: 39kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P06729 Background: CD2 is mainly present on the surface of mature T cells and NK cell membranes, while B cells are usually not expressed. It can be used to label the diagnosis and study of normal T cells and their associated tumors, such as pre-t cell lymphoma, marginal T cell lymphoma and anaplastic cell lymphoma. CD2 antibody is useful for identification of precursor and mature T-cell lymphomas. Aberrant loss of CD2 in T-cell lymphomas may help to distinguish them from reactive T-cell proliferations. Subcellular location: Membrane Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide corresponding to residues within N- Term of CD2 was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions:IHC-P: 1:100-1:200 Background References: 1. Companioni Nápoles O, et al. J Gastroenterol 52:39-49 (2017). 2. DE Sanctis V, Belgioia L, et al. ANTICANCER RESEARCH 39: 1935-1942 (2019). Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections analysis of T cell lymphoma tissue labelling CD2 with BP6107. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
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