CD14 Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50106 Clone# BP6111 Predicted Molecular Wt: 40kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P08571 Background: CD14 is receptor for endotoxin and apoptotic cells. It is expressed by monocytes/histiocytes, Langerhans’ cells, follicular dendritic reticulum cells, neutrophils (weakly), and endothelial cells of the liver sinusoids. CD14 is detected in myeloid leukaemias with monoblastic/monocytic differentiation, and in histiocytic neoplasms like histiocytic lymphoma, Langerhans’ cell histiocytosis and giant cell tumour. In cultures, a subpopulation of Kaposi sarcoma cells express CD14. In differential diagnosis, CD14 is more specific than CD68 in the identification of monocytes/histiocytes and associated neoplasias. Subcellular location: Membrane Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide corresponding to residues within aa1-100 of CD14 was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Scott CS, Eur J Haematol. 1990 Jan;44(1):24-9. 2. WrightSD, Science 1990;249:1431-3. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of appendix labelling CD14 with BP6111. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
杭州浩克生物
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