Human Placental Lactogen Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50109 Clone# BP6114 Predicted Molecular Wt: 25kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P0DML2 Background: Human placental lactogen (also called CSH1 or HPL), is a member of the human growth hormone (hGH)/human placental lactogen gene family. It is important in the regulation of maternal and fetal metabolism, as well as the growth and development of the fetus. Antibody to HPL reacts with syncytiotrophoblastic cells inchoriocarcinoma and intermediate trophoblasts in trophoblastic tumors. It is a useful marker for tumors with trophoblast differentiation. Subcellular location: Cytoplasm Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide corresponding to residues within aa100-200 of Human Placental Lactogen was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Walsh S.T., Kossiakoff A.A. J. Mol. Biol. 358:773- 784(2006). 2. Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D. Brief. Bioinformatics 12:449-462(2011). Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of placenta labelling Human Placental Lactogen with BP6114. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
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