Cytokeratin (HMW)兔单克隆抗体Cocktail

价格¥600
规格100ul
货号HKZ150234
单位支
品牌HaoKebio

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品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HKZ150234	Cytokeratin (HMW)兔单克隆抗体Cocktail	100ul	支	600

Cytokeratin (HMW)
Recombinant Rabbit Monoclonal Antibody Product Datasheet
Catalog# BX50111 Clone# BP6021/BP6077
Purity:
ProA affinity purified IgG
Species Cross-reactivity:
Human
Form:
Liquid
Applications:
IHC-P
Background:
In normal tissues, cytokeratin (high molecular weight) is mainly expressed in normal squamous epithelium, ductal epithelium and other laminated epithelium. Positive expression was found in lungsquamous cell carcinoma, breast cancer, pancreatic
cancer, cholangiocarcinoma and bladder transitional cell carcinoma.
Anti-cytokeratin, high molecular weight is capable of recognizing all basic keratins; therefore, it is a broadly reactive antibody staining most epithelia and their neoplasms. Members of the acidic and basic subfamilies are found together in pairs. Since
each epithelium contains at least one acidic and one basic keratin, this antibody is used to observe the distribution of keratin- containing cells in normal epithelia and to identify neoplasms derived from such epithelium.
Subcellular location:
Cytoplasm
Recommended method:
Heat induced epitope retrieval with Tris-EDTA buffer   (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
Immunogen:
Synthetic peptide corresponding to residues within
aa200-300 of  Cytokeratin 1 was used as an immunogen.
Synthetic peptide according to the aa490-590 (C-
terminal) of Cytokeratin 5 was used as an immunogen.
Storage Buffer:
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.
Storage conditions:
-25°C to -18°C
Storage instructions:
Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles.
Recommended Dilutions:
IHC-P:     1:100-1:200
Background References:
1. Kriho UK, et al. Virehows Arch. 1997; 431:139-47.
2. D Oh, RA Prayson. Arch Pathol Lab Med—Vol 123, October 1999:917-920.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded
sections) analysis of
esophagus carcinoma
tissue labelling Cytokeratin (HMW) . Heat mediated
antigen retrieval was
performed using Tris/EDTA buffer pH 9.0

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