Myo D1兔单克隆抗体

价格¥600
规格100ul
货号HKZ150243
单位支
品牌HaoKebio

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品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HKZ150243	Myo D1兔单克隆抗体	100ul	支	600

Myo D1
Recombinant Rabbit Monoclonal Antibody Product Datasheet
Catalog# BX50120 Clone# BP6124
Predicted Molecular Wt:
35kDa
Purity:   ProA affinity purified IgG
Species Cross-reactivity:
Human
Form:   Liquid
Applications:
IHC-P
Swissprot ID:   P15172
Background:
MyoD1, one of the MyoD family of myogenic helix-loop-helix transcription factors, combined with myogenin, plays a role in coordinating the myogenic differentiation pathway from the determination of mesodermal precursors into myoblasts, the
differentiation of myoblasts into myotubes, and finally the maturation of myotubes into skeletal myofibers. Normal mature     skeletal muscle does not express MyoD1 protein. MyoD1 is expressed in myoblasts before differentiation while myogenin has post-differentiation functions. Anti-MyoD1 immunostaining identifies cells committed to myogenesis in their earliest phase,    thus, it is a better biomarker for less differentiated Rhabdomyosarcomas (RMS) .
RMS are the most frequent malignant soft tissue neoplasms of childhood. While better differentiated RMS have cross-
striations or rhabdomyoblasts that allow for a confident morphologic diagnosis, less differentiated RMS resemble other small blue round-cell tumors.
Studies suggest, anti-MyoD1 may be used together with anti-myogenin and anti-desminas a panel of markers since any RMS is virtually never negative for all three markers simultaneously.
Subcellular location:
Nucleus
Recommended method:
Heat induced epitope retrieval with Tris-EDTA buffer   (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
Immunogen:
Synthetic peptide corresponding to residues within
aa1-100 of  Human Myo D1 was used as an immunogen.
Storage Buffer:
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.
Storage conditions:
-25°C to -18°C
Storage instructions:
Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles.
Recommended Dilutions:
IHC-P:     1:100-1:200
Background References:
1. Farup J  et al. J Appl Physiol (1985) 119:1053-63 (2015).
2.  Hoedt A  etal. J Physiol 594:727-43 (2016).
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded
sections) analysis of
Rhabdomyosarcomas
tissue labelling Myo D1 with BP6124. Heat
mediated antigen
retrieval was performed using Tris/EDTA buffer    pH 9.0

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