Mammaglobin Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50121 Clone# BP6125 Predicted Molecular Wt: 10.5kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: Q13296 Background: Mammaglobin, 10 kDa, is a cytoplasmic protein,a mammary-specific member of the uteroglobin family. It is related to a family of epithelial secretory proteins that includes prostatein and Clara cell protein. Mammaglobin occurs in about 80% of breast carcinomas. The extend is generally larger than that of GCDFP-15. Up to 15% of nonbreast carcinomas (such as stomach, lung, colon, hepatobiliary, thyroid, ovarian, and urothelial carcinomas) have been reported positive, usually only focally. Mammaglobin is a sensitive and fairly specific marker for breast carcinoma. The panel should also include Gross cystic disease fluid protein-15 and Estrogen receptor alpha. Subcellular location: Cytoplasm Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Recombinant fragment corresponding to Human Mammaglobin was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Fanger GR etal. Tumour Biol 23:212-21 (2002). 2. Han JH etal. Arch Pathol Lab Med 127:1330-4 (2003). Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of breast carcinoma labelling Mammaglobin with BP6125. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
杭州浩克生物
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